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HTS Breakthroughs Powered by MCE Libraries
On the challenging and lengthy journey of scientific research, who wouldn't want their research results to advance as swiftly as a rocket? At this point, one must not miss high-throughput screening, a magical existence that can be called the "super accelerator" in the scientific research field! It's like equipping the research process with a powerful turbocharged engine, significantly speeding up drug screening and mechanism verification. Today, We had organized some high-score cases of high-throughput screening using the MCE compound library, let's delve into them together and learn!
By MedChemExpress4 months ago in Trader
Ultrasound-Triggered Cuproptosis: A Novel Strategy for Targeted Cancer Therapy
Inducing precise and efficient cancer cell death while stimulating an immune response remains a critical focus in oncology. A recent study published in Advanced Materials introduces a novel approach: ultrasound-triggered nanoparticles (RC NPs) that, for the first time, integrate cuproptosis with sonodynamic therapy (SDT). This innovative platform offers a spatiotemporally controlled and immunoactivating strategy for treating pancreatic and other aggressive cancers.
By MedChemExpress5 months ago in Trader
Detailed Protocol for CCK-8 Assay
01 Basic Experiment: CCK8 Assay Cell Counting Kit-8 (CCK-8) is a colorimetric assay kit widely used for the rapid, highly sensitive, and non-radioactive detection of cell proliferation and cytotoxicity, based on WST-8. The CCK-8 solution can be directly added to cell samples without the need for pre-preparation of various components. In the presence of electron coupling reagents, WST-8 can be reduced by certain dehydrogenases in the mitochondria to generate an orange-yellow formazan (Figure 1). The more and faster the cells proliferate, the darker the color; the greater the cytotoxicity, the lighter the color. For the same type of cells, there is a linear relationship between the color intensity (amount of formazan produced) and the cell number (Figure 2).
By MedChemExpress6 months ago in Trader
Study Sharing! How do MCE's clients conduct drug discovery?
High-throughput screening is well known as an efficient method for drug discovery and disease mechanism researches. But how to carry out drug screening experiments? How to guarantee the correctness of the lead compounds that screened out? Let's take a look at how high-throughput screening is carried out by using two papers as references.
By MedChemExpress6 months ago in Trader
Cell Migration vs. Invasion: Differences Revealed by Scratch Assays and Transwell Experiments
Cell Migration Cell migration (also known as cell crawling, cell movement or cell motility) refers to the movement of cells in response to migration signals or the gradient of other substances. During this process, the cell extends protrusions at the front, forms new adhesions, and alternates between the front and rear of the cell by contracting the tail region in both space and time. Cell migration is a common form of movement in living cells, playing a crucial role in important biological processes such as embryonic development, wound healing, immune response, and tissue remodeling[1][2].
By MedChemExpress6 months ago in Trader
Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!
Studies of different molecules in nature have revealed that the formation of carbon-heteroatomic bonds is superior to the formation of carbon-carbon bonds. Nucleic acids, proteins and polysaccharides are condensation polymers connected by carbon-heteroatom bonds. Sharpless was inspired by this and put forward the concept of "click chemistry" in 2001. Click chemistry is the rapid chemical synthesis method of useful new compounds through heteroatom connection (C-X-C), which is used to describe selective, modular, wide range and high yield chemical reactions[1][2].
By MedChemExpress7 months ago in Trader
Details of basic cell culture methods and operation steps
Preparations for receiving cells 1. Recording Key Cell Information When receiving a cell line in the lab for the first time, several pieces of information related to the cell line should be organized and recorded. These details are crucial for ensuring successful cell propagation, expansion, cryopreservation, and storage. It is strongly recommended to document the following information before starting cell expansion: (1) Background information (2) Subculturing (3) Cryopreservation (4) Cell line storage.
By MedChemExpress8 months ago in Trader
Details of basic cell culture methods and operation steps
Preparations for receiving cells 1. Recording Key Cell Information When receiving a cell line in the lab for the first time, several pieces of information related to the cell line should be organized and recorded. These details are crucial for ensuring successful cell propagation, expansion, cryopreservation, and storage. It is strongly recommended to document the following information before starting cell expansion: (1) Background information (2) Subculturing (3) Cryopreservation (4) Cell line storage.
By MedChemExpress8 months ago in Trader
Common cardiovascular disease models and modeling schemes
Hypertension Models 1. Genetic Models of Hypertension Genetic hypertension models can simulate the genetic phenomena in humans, among which the spontaneously hypertensive rat (SHR) is the most commonly used.
By MedChemExpress8 months ago in Trader
How they used PKH 26 for cellular studies?
With the increasing trend of global aging, sarcopenia has become a significant public health issue. Sarcopenia, a loss of skeletal muscle mass and function associated with aging, is a progressive and widespread skeletal muscle disease.
By MedChemExpress10 months ago in Trader
