kiko Garcia
Bio
CD Genomics is a leading global life sciences company, and we remain committed to providing the research community with high-quality long-read sequencing services. snp genetics
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Variant Calling: From Sequence Data to Reliable Mutation Detection
Variant calling is a pivotal process in genomic research, enabling the identification of genetic differences between an individual's genome and a reference genome. This article provides a comprehensive overview of the best practices for variant calling in clinical sequencing, covering data preprocessing, alignment, variant detection algorithms, filtering, and validation. It also discusses the challenges and future directions in this rapidly evolving field, emphasizing the importance of rigorous methods and benchmarking to ensure reliable mutation detection.
By kiko Garcia6 months ago in Writers
Introduction, Methods, and Advantages of Microbiome RNA Sequencing
Introduction to Microbial Profiling using 16s rRNA Sequencing The use of 16S ribosomal RNA (rRNA) sequencing for microbial profiling is a common method for studying bacterial phylogeny and taxonomy. Because it contains both highly conserved and hypervariable regions, the 16S rRNA gene is the most widely used genetic marker for bacterial identification and classification. The conserved regions can act as universal primer binding sites for amplification of whole genes or gene fragments, whereas the hypervariable regions contain species-specific sequences that can distinguish between bacteria and archaea. Similarly, for taxonomic profiling of fungi, the internal transcribed spacer (ITS) region is used.
By kiko Garciaabout a year ago in BookClub
RNA Splicing and How to Target It
What Is RNA Splicing? RNA splicing is a post-transcriptional modification process that occurs in eukaryotic cells. When a gene is transcribed, the initial RNA product is called pre-mRNA, which contains both coding regions (exons) and non-coding regions (introns). RNA splicing removes the introns from the pre-mRNA molecule and joins together the exons to form a mature mRNA molecule that can be translated into protein.
By kiko Garciaabout a year ago in BookClub
Introduction, Workflow, and Analysis Pipelines of RNC-RNA Sequencing
When a ribosome is affixed to the polypeptide (protein) it is making, the ribosome-nascent chain complex (RNC) is the compilation of molecules that make up the ribosome. One of several methods can be used to halt the synthesis of the nascent polypeptide. RNCs are made and purified in labs to study the dynamics, biochemistry, folding, and interactions that the ribosome and proteins undergoing synthesis.
By kiko Garciaabout a year ago in Earth
A Guideline of Genetic Map Construction for Different Populations
What is Genetic Map? Genetic map construction for different populations is a fundamental process in plant genetics research, allowing us to gain insights into the arrangement and linkage of genes on chromosomes. Also referred to as a genetic linkage map, it is built by analyzing the connections between genetic markers within a population.
By kiko Garciaabout a year ago in Earth
